Okay... I guess I should be more specific... BRASS barbs of that type. The plasic ones should be okay as they are a softer material and will compress some as opposed to cutting into your tubing.
Mine are all brass of the "bad" type with NO clamps on the blocks and simple Acrylic straight tubing on the reservior and the elbows I made. These are larger than the inner diameter of the tubing and so the tubing has to stretch over it and is VERY tight, also with no clamps.
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MMZ>TimeLord
"Oooooooooh... that's gonna leave a mark!"
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