Hi Pears0, and welcome to the forums!
Depending on the block material(s), you got various sealing materials to choose from - goop, bathroom silicone, liquid sealants, glue, epoxy, O ring, etc etc... the choice is yours, dependant on the materials you use.
The layers are usually fasten together with bolts or in case of full copper block, bolts or solder.
It would be easier to answer if you state your choice of materials and be more precise about the whole block...
Cheers!
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